A Colorimetric Micromethod for the Estima- Tion of Cystine and Cysteine”
نویسنده
چکیده
Fleming’s (1) observation that cystine in the presence of ferric ions forms a blue color when heated with p-aminodimethylanilinc suggested the application of this reaction to a quantitative estimation of cystine in ,biological materials. The formation of the blue color occurs in strongly acid solution, which enhances the value of the reaction as a means of estimating cystine in the acid hydrolysates of proteins. Moreover, the simplicity of the method outlined below lends itself to routine applications, an advantage over the procedure of Fujita and Numata (2).
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